In this Part 2 of a series I again delve into some technical detail, but this time in an effort to catch a little bit of the form and movement, the dynamism, of genetic and epigenetic processes. I well realize that the material in this series will present a challenge to many readers. The presentation is both denser and lengthier than normal for NetFuture. I've been motivated to produce these articles by the awareness that epochal changes in biology are underway, and that the popular media have so far grossly failed to give the public any realistic feel for what is happening. A reasonably detailed and generally accessible summary of the ongoing, highly technical research needs to be available somewhere.
The readership for such material may be rather more limited than for the general run of NetFutures, but I consider this readership extremely important. And you can help me out in one regard: if you find these articles of use, please drop me a line to let me know. I will also welcome any suggestions for improvement.
I am currently expecting that there will be a total of four articles tracing some of the current research relating to epigenetics and gene regulation. These will be followed by attempts to draw out the significance of the work for biology, for science in general, and for the controversial notion of "holism". I do believe we are headed toward a revision of the scientific outlook that may be the equal of any of the great "paradigm shifts" of the past — this despite the fact that penetrating discussion of the implications of the current work has scarcely even begun. It's almost as if no one — or, at least, no one who is attempting to publish in the primary, peer-reviewed journals — has quite dared as yet to step outside the old rhetoric of "genetic code", "mechanism", "building blocks", and all the rest, and actually face what is going on.
On a different note: if you are interested in evolution, you might want to check out two articles in the latest issue of The Nature Institute's hardcopy newsletter, In Context. These articles have been posted to the web.
Go to table of contents
(You will find the latest versions of the currently available parts of this series at the website, "From Mechanism to a Science of Qualities".)
The decades following the 1953 discovery of the double helix were a time when everything important seemed to hinge on the fixed and definitive "genetic code". The researcher's task was to work out explicitly a prescriptive logic already contained in the code. It was not a time when molecular biologists were likely to preface their discussions of gene expression with statements like this:
DNA is a living molecule, writhing, twisting and bending in response to the physical forces applied to it by genetic processes (Kouzine et al. 2007).Nor was it a time when gene packaging materials and a diverse bestiary of regulatory factors would have been described, with today's peculiar emphasis, as participants in a "delicate 'dance' in time and space", a "regulatory ballet", "an intricate dance of associations", and a "chromatin choreography". Nor had there yet occurred that strange increase we now observe in the use of terms such as "balance", "tension", "context", and "plasticity", together with an exponential explosion during the first decade of the twenty-first century in the appearance of the word "dynamic" in reference to the chromosome, chromatin remodeling proteins, nucleosomes, and the spatial organization of the nucleus — all pointing (in the words of two biologists) toward the "highly choreographed" events regulating gene expression, a "three-dimensional pavane . . . that controls our genome" (Fraser and Engel 2006).
Or, again, as recently as 1996, when the yeast genome was fully decoded and all its secrets supposedly laid bare, who would have expected to read this a decade further on:
Even for a [yeast] genome that has been studied intensively since it was sequenced 10 years ago, a glimpse into the complexity of its transcriptional architecture makes this genome appear like novel territory (Lior 2006 et al.).
How could the relatively simple yeast genome remain novel ten years after the complete unveiling of its structural sequence? And as for the choreography of the cellular "dance", isn't this a mere metaphor? Surely the authors quoted above did not really mean to say that a dance controls the genome! After all, the decisive logic of the genomic code is supposed to be what ultimately controls everything else.
Or so it has been thought. But the pace of discovery today is almost blinding, and it seems to be a time for the loosening of old thought patterns. The appeals to genomic plasticity, balance, tension, and context, the language of dynamism and artistic movement — these are not mere signs of a collective weakness for poetic invention, but rather are evoked by the phenomena under consideration. There is, in fact, nothing to prevent the contemporary molecular biologist from thinking along the following lines:
The organism must be rooted in something more than an abstract, fixed, and unchanging logic. It is a dynamic material presence, and requires a materially effective genesis. If DNA and all the other contents of the living cell provide the physical foundation for the organism as a whole — for the seeing eye, the beating heart, the graceful and compelling movement of the ballet dancer — then why shouldn't their own, molecular performance be at least as artful and complex as that which they support on a larger scale? Why shouldn't their gestures be as meaningful and expressive as those of the organs whose development they so effectively underwrite? Is there any reason to think that the life animating the cell and its DNA should be any more reducible to a static logical sequence — should be any less subtle or capable or dynamic — than eye, heart, and ballerina? Why should we project tiny, neatly programmed mechanisms into the cell when the organisms we see don ’t at all look or behave like such mechanisms?
Of course, these thoughts, especially if expressed in this way, would still today appear rather eccentric. There are, as we will see, logic-centered and mechanistic habits of thought that fiercely oppose giving embodied (and therefore observably aesthetic) form and movement their due.
But nevertheless, the landscape on which we all must move with our ideas, outworn or forward-looking as they may be, is inexorably changing. It's a landscape upon which, less than a decade after the height of the fever induced by the Human Genome Project, the author of a review in a major biological journal could write that genome mappings and genomic comparisons of species "shed little light onto the Holy Grail of genome biology, namely the question of how genomes actually work" in living organisms (Misteli 2007).
And so today whoever would take a researcher to task for eccentric thinking might have a harder time of it than usual. In surveying contemporary molecular biology, the least anyone can say is, "Things are getting very interesting!"
If you arranged the DNA in a human cell linearly, it would extend for about two meters. How do you pack all that DNA into a cell nucleus about ten millionths of a meter in diameter? According to the usual comparison it's as if you had to pack 24 miles (40 km) of extremely thin thread into a tennis ball. Moreover, this thread is divided into 46 pieces (individual chromosomes) averaging, in our tennis-ball analogy, over half a mile long. Can it be at all possible not only to pack these into the ball, but also to keep them from becoming hopelessly entangled?
Let's begin visualizing the situation in a little more detail.
Imagine you have a rope consisting of two strands spiraling around each other in the manner of a double helix. Imagine further that there are short, fairly rigid rods connecting the two strands at regular intervals along their entire length. There are many of these rods — roughly ten along the brief length required for one strand to spiral completely around the other a single time. Each rod represents two linked nucleotide bases, or base pairs (complementary "letters" of the genetic code), with one base bound tightly to one strand of the rope and the other bound to the complementary strand.
It would be good if the rope, in its double helical form, has been soaked in a starch solution or some other stiffening agent. This is because DNA, as a result of its chemical constitution, possesses a degree of natural rigidity; left to itself, the overall structure resists bending, and one strand "wants" to wrap around the other a fixed number of times for any given length of the helical axis. It is possible to increase this number somewhat (that is, to wind the strands more tightly around each other) or else to decrease it (unwind the strands). But in both cases this is to work against the stiffness of the natural structure, and therefore to create tension that must be accommodated in one way or another.
You have doubtless seen this accommodation many times. Hold the ends of a two-stranded rope in your hands and begin to twist one of the ends so as to tighten the spiraling strands. Before long you will find the rope coiling into something like a figure eight, and then into ever more complex forms as you continue twisting, until finally you end up with a "nest of worms". And much the same happens if you twist in the opposite direction, as if you were trying to unwind or loosen the spiraling strands. The coil resulting from a tightening twist is called a positive supercoil, while a loosening twist produces a negative supercoil1.
The forces involved in these deformations can be very large — as you will have discovered if you have ever tried to coerce a stiff rope through multiple stages of coiling by twisting its ends, or even if you have found yourself wrestling with a recalcitrant garden hose while trying to coil it neatly. Matter can be very resistant! Analogous forces come into play with chromosomes as well.
DNA is often in a negatively supercoiled state to one degree or another — and, as we will soon see, this is owing to additional reasons beyond the fact that, in order to fit into its own space in the nucleus, it is coiled, bent, wound and otherwise structured to an almost unfathomable degree. The question is how any sort of order is maintained: how is the nest of worms packaged and managed in every cell of the human body in a manner allowing the thousands of distinct, individual genes, and perhaps hundreds of thousands of regulatory sequences, to come to harmonious expression within the complex life of the larger cell?
The first step in DNA packaging involves tiny "spools" made of histone proteins — some thirty million of them in the human genome, so that there can be several hundred thousand or more in a single chromosome. The DNA double helix commonly wraps about two times around this spool, continues on for a short distance, then wraps around a second spool, and so on. The DNA-enwrapped spool is called a nucleosome.
This first level of DNA packaging is often described as "beads on a
string". (See third image from left in the figure below.) The DNA and
histone spools, together with numerous other attached proteins and smaller
chemical groups, give an overall, ever-changing form and structure to what
is called chromatin— the
actual material of the chromosome. But with this spooling of the double
helix we have seen only the beginning of the compaction that must occur in
order to fit the chromosome into the cell nucleus. Unfortunately, the
higher-order structures of the compacted chromosome are still little
understood. The spools with their DNA somehow get packed into dense,
three-dimensional arrangements, and this entire arrangement coils further
upon itself beyond anyone's current ability to unravel the details. Such
difficulty, however, rarely hinders the adventurous from offering visual
models. So, for what it is worth, here is a conventional picture showing
several stages in the condensation of a chromosome (it is best viewed at
full screen width):
For credits and permissions, see http://upload.wikimedia.org/wikipedia/commons/4/4b/Chromatin_Structures.png.
During the cell's normal functioning the chromosome is not as fully condensed as it is during cell division (the two images at far right). Nor is it all in one state. Some parts of it — especially the parts containing many active genes — are in something rather more like the "beads-on-a-string" form, while other parts may be in the conformation of the 30-nanometer fiber, and vast regions are in a much more wound-up form. (Thirty nanometers is 30 billionths of a meter, or about 3 thousandths of the diameter of a typical cell nucleus.) In general — but with exceptions — the more compact the chromatin, the less available are the genes for transcription.
Another image follows below, this one showing four proposed models —
each viewed from two different angles — for the structure of
chromatin in the 30-nanometer fiber. The models do not show the actual
spools or other proteins, but only the DNA. (The DNA is given as a simple "wire",
without any representation of the two helical strands.) However, you can
see how one spool could be positioned inside each double spiral of DNA.
Then, given the scale of the image, you would need to picture this
arrangement extending linearly for enormous distances, even if only a
small part of a chromosome were represented.
Graphics by Julien Mozziconacci (http://en.wikipedia.org/wiki/File:ChromatinFibers.png)
Linker DNA — the short, connecting lengths of DNA between spools — is shown in bright yellow, and the wrapped DNA is flesh-colored. The different models are based on different assumptions about the total number of base pairs from the start of one spool to the start of the next one — that is, the length of wrapped DNA plus linker DNA. These lengths are the numbers shown in the figure. You can bring the upper and lower images into proper relation if you imagine each of the upper images rotated ninety degrees around a horizontal axis so as to bring the brightly colored (blue, pink, green, or gold) double spiral of the upper image into the position shown in the lower image. Finally, the white "lumps" in the figure represent linker histones, which hold the DNA to the spool and help to stabilize the entire array.
Perhaps none of this helps us greatly to understand how the extraordinarily long chromosome, tremendously compacted to varying degrees along its length, can maintain itself coherently within the functioning cell. But here's one relevant consideration: there are enzymes called topoisomerases, whose task is to help manage the forces and stresses within a chromosome. Demonstrating a spatial insight and dexterity that might amaze those of us who have struggled to sort out tangled masses of thread, these enzymes manage to make just the right local cuts to the strands in order to relieve strain, allow necessary movement of individual genes or regions of the chromosome, and prevent a hopeless mass of knots.
Some topoisomerases cut just one of the strands of the double helix, allow it to wind or unwind around the other strand, and then reconnect the severed ends. Other topoisomerases cut both strands, pass a loop of the chromosome through the gap thus created, and then seal the gap again. (Imagine trying this with miles of string crammed into a tennis ball — without tying the string into knots!) I don't think anyone would claim to have the faintest idea how this is actually managed in a meaningful, overall, contextual sense, but great and fruitful efforts are being made to analyze isolated local forces and "mechanisms".
Before we try to bring the picture a little more alive, there's one small exercise that may help us. Many window shades have a looped cord for adjusting the light. If you slip your finger through the loop at the bottom and then twist it around in one direction many times, you will get our familiar double-stranded helix. Now, while keeping firm hold of the loop at the bottom, insert a pencil between the strands near the near the middle of the cord's length and then force the pencil downward. You will observe that the stands become progressively more tightly wound beneath your pencil, until it can move no more. At the same time the cord above the pencil becomes more loosely wound. Alternatively, you can let go the loop at the bottom, in which case the cord will spin around as the pencil descends.
This is relevant to the chromosome because when a gene is transcribed, its two double helical strands need to be separated, or "unzipped", as the transcribing enzyme moves along. How, then, does the chromosome accommodate the twisting forces imposed by this local "unzipping" of its two strands? You might expect the chromosome to spin like the cord with a pencil moving down it. Certainly there is some such movement. But if it were to proceed in an unconstrained manner, as with the released window shade cord, the entire chromosome ahead of the transcribing enzyme would have to make about 2850 complete turns during transcription of an average-sized gene (Lavelle 2009), which means rotating at several turns per second. Clearly, given the length, the mass, and the complex bending and looping forms of the chromosome, and given the extremely thick "soup" of macromolecules in the cell nucleus, such movement would be greatly impeded.
Furthermore, the ends and many points within the chromosome are typically "fastened down", as we will see later, so there isn't all that much freedom of movement. Of course, when you move the pencil down between the two strands of the window cord, you could allow the pencil itself to spin. This would leave the helical structure of the cord mostly unchanged outside the immediate vicinity of the pencil. However, in the cell our "pencil" — the transcribing enzyme — is part of a very large molecular complex. In addition, it is associated with a cumbersome set of proteins for disassembling nucleosomes ahead of its transcribing activity, reassembling them behind, and performing various other tasks. And it is attached to the ever-lengthening strand of RNA that it is itself producing. So it faces limits upon its mobility similar to those of the chromosome.
The upshot of it all is that there are many complex movements, highly constrained and absorbed in varying ways by the different resistant elements of the complex structures involved. In general, positive supercoiling occurs ahead of the transcribing enzyme's "unzipping" action and negative supercoiling behind it. Topoisomerases play their role in managing both the stresses and the overall conformation of the chromosome "tangle", as do many other poorly characterized players in the sculptural drama of form and force that is the chromosome.
I have so far described the packaging of human DNA as a mere technical challenge. That's a big problem. The tensions and movements, the bending and unbending, the coiling and uncoiling, are much more than the expression of mechanical forces aimed at chromosome condensation. It was quite wrong of me to begin by asking you to imagine twisting a rope since, after all, there is no one — no specific agent — in the cell nucleus performing this task. The chromosome is not a passive, limp object moved only from outside. Interacting with its surroundings, it is as much a living actor as any other part of its living environment. Maybe instead of a rope, we should think of a snake, coiling, curling, and sliding over a landscape that is itself in continual movement.
The chromosome, in other words, is doing something. It is engaged in a highly effective spatial performance. It's movements are not simply the result of its being packaged and kept out of trouble, but rather are well-shaped responses to sensitively discerned needs. These movements bear decisive significance for the life of the cell and organism as a whole. Far better to picture the chromosome as both a sensing and muscular presence than as a rope.
To begin with, the mechanical stresses induced by transcription are now known to contribute broadly to gene regulation. "The organization of global transcription is tightly coupled to distribution of supercoiling sensitivity in the genome" (Blot 2006). Increases in twist (positive supercoiling) are associated with chromatin folding and gene silencing in the supercoiled region, whereas decreases of twist (negative supercoiling) are associated with "acquisition of transcriptional competence" (Travers and Muskhelishvili 2006). Moreover, "negative supercoils are dynamic. The slithering and branching of the interwound strands allow DNA to act like a chaperone, promoting the long-range assembly and disassembly of protein-DNA complexes" (Deng et al. 2005) — complexes that play a vital role in gene regulation. Each type of cell has its own characteristic patterns of supercoiling, which is doubtless related to the fact that it also has its own distinctive patterns of gene expression. Christophe Lavelle of the Curie Institute in France summarizes the recent research findings this way:
As DNA is rotating inside the polymerase [transcribing enzyme], positive and negative supercoiling is induced downstream and upstream, respectively. Transcriptionally generated torsion, rather than a mere waste product to be disposed of by topoisomerases, has instead recently been shown to propagate through the chromatin fiber and trigger local DNA alterations, detected as a regulatory signal by molecular partners. (Lavelle 2009)
To illustrate the regulatory possibilities: researchers at the National Cancer Institute in Bethesda, Maryland, found that negative supercoiling upstream (behind) a transcribing enzyme was sufficient to cause a local, nonstandard conformation of the double helix, which in turn enabled recruitment of regulatory proteins sensitive to such changes in structure (Kouzine et al. 2008).
So the chromosome's twisting and writhing is not merely arbitrary; it is sculpturally significant movement, carrying meaning for the chromosomal stretches along which it is communicated.
But there are many other dimensions of the chromosome's spatial performance. Each chromosome has its own preferred territory within the nucleus and its preferred neighbors, which also differ from one cell type to another. These territories "are dynamic and plastic structures" that "can be dynamically repositioned" (Schneider and Grosschedl 2007). Since living conditions are close, the neighbors matter. A chromosome's territory appears to be shaped rather like an irregular potato or a sponge. There is at least some socializing between adjacent chromosomes, with protrusions of one territory penetrating into the hollowed-out portions of the next territory and even of more remote territories (Ling et al. 2007). So not only are distantly separated portions of the same chromosome brought into intimate contact by the geometry of the sponges, but loci on separate chromosomes can also be brought into contact.
It happens that both sorts of contact have a great deal to do with gene expression. On an earlier view, the DNA sequences regulating a protein-coding gene were always close to the gene or at least not very far removed. But in more recent years it's been recognized that some regulatory sites — "enhancers" and "silencers" and "locus control regions"— may be located on distant parts of the chromosome, thousands or hundreds of thousands or even millions of base pairs away from the gene being regulated. Expression is enabled, for example, when the distant enhancer is brought into physical proximity with the gene or genes it regulates. (Another remarkable feat of contextually apt physical coordination!) In connection with this, an activator protein is bound to the enhancer and then, perhaps in concert with one or more co-activators, may assist in constellating the massive transcription complex on the gene's promoter sequence.
A locus control region (LCR) is a DNA sequence that helps to regulate a cluster of related genes. One research team in the Netherlands, working with mice, examined an LCR for a set of genes relating to the production of beta-globin (a constituent of hemoglobin). In fetal liver tissue, where these genes are highly active, the LCR was found to associate with dozens of genes, including many involved in beta-globin production. Some of these genes were tens of millions of base pairs distant on the chromosome. Further, in fetal brain tissue, where the beta-globin genes are inactive, the LCR again associated with many other sites — but now a completely different set. The researchers concluded:
Our observations demonstrate that not only active, but also inactive, genomic regions can transiently interact over large distances with many loci in the nuclear space. The data strongly suggest that each DNA segment has its own preferred set of interactions. This implies that it is impossible to predict the long-range interaction partners of a given DNA locus without knowing the characteristics of its neighboring segments and, by extrapolation, the whole chromosome. (Simonis et al. 2006)
It's not only where loci on a single chromosome are brought into contact with each other that they can interact, however. Increasing numbers of cases are being reported where contact between sites on different chromosomes plays a crucial role in gene regulation. In fact, the mouse study just cited demonstrated a number of such contacts. (A few researchers began to speak of "kissing chromosomes" — a not very helpful phrase that seems now to have been dropped from the literature.) Here's a schematic representation of one case of interchromosomal interaction. (Skip the next three paragraphs if you don't want to bother with the technical details.)
From Schneider and Grosschedl 2007.
"T helper" or TH cells are human immune system cells. One type of TH cell (TH1) produces, among other things, interferon (IFN-gamma), while a second type (TH2) produces various interleukins such as IL-4 and IL-5. But before a cell becomes either a TH1 or TH2, it resides in a less differentiated state as a "naive" TH cell (referred to as a "T cell" in the figure). Only after stimulation by an antigen (a substance that provokes formation of antibodies), does the TH cell become either a TH1 or TH2 cell.
If you look at the genes responsible for producing interferon in TH1 and interleukins in TH2 cells, you find the usual suspects: the interferon gene on chromosome 10 seems to be regulated by nearby genomic elements, while the interleukin genes on chromosome 11 are also regulated by local sites — in particular, by an LCR. But a research team at the Yale University School of Medicine decided to investigate the larger spatial picture. They discovered that regulatory regions associated with the interferon and interleukin genes, despite being on separate chromosomes, were physically close together in naive cells. Upon stimulation of naive cells by an antigen, the "negotiations" between these regulatory regions somehow determined which of the genes would be active and which would be repressed — and therefore whether the cell would become a TH1 or TH2 cell. Following this determination, the two chromosome regions moved apart.
The illustration (right side) shows the case where a TH2 cell has resulted. Interferon (IFN-gamma) is not expressed (upper right) because the looping pattern separates the requisite gene from its enhancer. On the other hand, IL-5 is expressed (lower right), because the protein SATB1, which plays a large role in chromatin organization and transcription regulation, has anchored a series of chromosome loops in just such a way as to bring the IL-5 gene and Rad50 promoter into proximity with the locus control region. (Spilianakis et al. 2005; see also commentary in Kioussis 2005.)
Of course, as researchers dealing with this sort of thing readily acknowledge, questions abound. What guides particular sites on two different chromosomes to their rendezvous, and what sees to their subsequent separation? One could imagine, in the case of distantly separated sites on the same chromosome, that a regulatory protein binds to the one locus and then "tracks" along the chromosome until it finds the second locus (which it must have some way to recognize as significantly related to the first). But it's not at all easy to picture what it is that selects and brings together many loci on different chromosomes.
As is evident from the case of TH cells, chromosome looping can keep sites apart as well as bring them together. It not only serves the purpose of expression, but also of repression. In a study of red blood cells, a group of scientists from Children's Hospital in Philadelphia showed that successive stages of cell maturation were marked by different proteins playing a direct role in reconfiguring chromosome loops — first for expression of a particular gene, and later for repression (Jing et al. 2008). In general, chromosome loops help to make possible the more or less independent regulation of different gene regions — an important role in an environment thick with diverse regulatory factors and processes.
But how does a locus on a chromosome "take off" through the three-dimensional space of the nucleus, uncoiling from a more condensed state into a thin thread and looping outward from its territory for considerable distances, as if drawn by an invisible hand toward a rendezvous with a distant location? One group of researchers positioned a transcriptional activator on a particular chromosomal site located close to the periphery of the cell nucleus. Within 1 - 2 hours, the site migrated to the interior of the nucleus, following a curvilinear path roughly perpendicular to the nuclear envelope. The movement, which was interspersed with several-minute periods of quiescence, reached a maximum velocity of about 1/10 the nuclear diameter per minute. These results led the researchers to speak of "fast and directed long-range chromosome movements" (Chuang et al. 2006).
A fairly recent surprise has been the discovery of actin and myosin in connection with some chromosome movements. These two substances, which play a major role in the contraction of muscles, seem also to provide a kind of "musculature" within the nucleus. Get rid of them, and certain observed movements stop. But little is yet known about how the movement is actually achieved, and even less about how it is directed.
What is now known, however, is that the nucleus is much more than a linear assembly line for the construction of proteins based on genetic sequences. It participates in an elaborately organized, three-dimensional space, and the positioning and movement of both chromosomes and the regulatory elements within the nucleus have everything to do with the functioning of the genome.
But while extraordinary research energies are now directed toward articulating the undeniable structural organization of the cell nucleus in its relation to gene regulation, an overall, coherent picture of the organization remains elusive. The titles of several articles currently lying on my desk point to the challenge investigators face:
"Dynamics and Interplay of Nuclear Architecture, Genome Organization, and Gene Expression" (Schneider and Grosschedl 2007).
"Dynamic Genome Architecture in the Nuclear Space: Regulation of Gene Expression in Three Dimensions" (Lanctôt et al. 2007).
"The Third Dimension of Gene Regulation: Organization of Dynamic Chromatin Loopscape by SATB1" (Galande et al. 2007).
"Dynamic Regulation of Nucleosome Positioning in the Human Genome" (Schones et al. 2008).
"Dynamic Organization of Gene Loci and Transcription Compartments in the Cell Nucleus" (Spudich 2008).
"Nuclear Functions in Space and Time: Gene Expression in a Dynamic, Constrained Environment" (Trinkle-Mulcahy and Lamond 2008).
You will have noted the repeated juxtaposition — spatial
organization on the one hand, dynamism on the other. How does one capture
organization that is dynamic and ever-shifting? The question only becomes
more acute when we look at a few additional aspects of nuclear
organization, as currently described in the literature:
Chromosome Domains. Chromosomes, as we have seen, participate in the highly structured space of the nucleus. But that is not all. They themselves are structured along their length, being subdivided by various means and in ever-changing ways into chromosome domains. We've already seen the organization of the chromosome into densely compacted regions (known as heterochromatin) and less condensed, more active regions (euchromatin). The boundaries between such regions are not always well-defined. Simply by residing close to a region of heterochromatin, a gene that otherwise would be very actively transcribed might be only intermittently expressed, or even silenced altogether. Where somewhat more cleanly separate regulation of neighboring loci is important, special DNA sequences called insulators can help prevent the "leaking" of influence from one region of the chromosome to the next.
Chromosome domains are also established by the twisting forces (torsion) communicated more or less freely along bounded segments of the chromosome. (The boundaries might be defined, for example, by the tethering points of chromosome loops.) The loci within such a region share a common torsion, and this can attract a common set of regulatory proteins. The torsion also tends to correlate with the level of compaction of the chromatin fiber, which in turn correlates with many other aspects of gene regulation. And even on an extremely small scale, the twisting (by linker histones) of the short stretches of DNA between nucleosomes— or the untwisting brought about by the release of the histones — is presumed to drive the folding or unfolding of the local chromatin (Travers and Muskhelishvili 2006). All this reminds us that gene regulation is defined less by static entities than by the quality and force of various movements.
There are still other ways that the chromosome reveals itself as a dynamic, complexly structured context. Genes expressed in the same cell type or at the same time, genes sharing common regulatory factors, and genes actively expressed (or mostly inactive) tend to be grouped together. One way such domains could be established is through the binding of the same protein complexes along a region of the chromosome, thereby establishing a common molecular and regulatory environment for the encompassed genes. But it's important to realize that such regions are more a matter of tendency than of absolute rule. A few examples from a summary by Elzo de Wit and Bas van Steensel at the Netherlands Cancer Institute illustrate the situation:
These rough tendencies do not enable precise predictions, but yet the
tendencies are really there; they point toward meaningful organization,
even though the observed "rules" are less the determiners of that
organization than they are the continually modified products of it. This
is exactly what you would expect in any living context, where a larger
unity — the unity that leads us to refer quite naturally to a
living being — shapes the activity of local parts and
processes to its own intention.
The "Pull" of the Nuclear Periphery. There is a fibrous network — the "nuclear lamina" — located primarily at the inside face of the nuclear envelope. In vitro studies (what used to be referred to as "test-tube studies") have shown that several proteins of this network can interact with chromatin. And now, as the Dutch biologists summarize, it has been found that there are more than 1300 lamina-associated chromosome domains (LADs) in the human genome that do indeed preferentially locate themselves at the nuclear periphery. De Wit and van Steensel (2009) mention studies showing that the artificial anchoring of a chromosome locus to the nuclear lamina "can cause partial downregulation of some (but not all) genes surrounding the anchoring sequence".
There seems to be a general rule that the chromosomes and chromosome
territories located toward the periphery of the nucleus are less
transcriptionally active and
also less gene-dense. Conversely, the nuclear interior shows higher rates
of activity and greater gene density. Researchers can activate genes near
the nuclear envelope and then watch them as they move toward the interior.
Likewise, they can silence genes in the
interior and watch them relocating to the periphery. Nevertheless,
transcription does occur in the outlying regions, and silent regions of
chromosomes reside in the interior. And, as always, multiple dimensions
of regulation work together. For example, the radial positioning of genes
seems to be connected to specific histone modifications of the sort
we looked at in Part 1 — although it's a matter of rough rather than
absolutely consistent correlation (Strašák et al. 2009).
Nuclear Matrix. It is not only the peripheral nuclear lamina that provides a kind of skeletal structure for organizing the nucleus. There is, throughout the nuclear space, a still poorly characterized and elusive "nuclear matrix" — so elusive that its fundamental nature is still debated. The nuclear lamina can be considered part of it, and there are many other substances that seem to play a role, including the SATB1 protein we encountered in connection with looping chromosomes, a topoisomerase, actin, and even the DNA transcribing and replication enzymes. Many of the proteins that associate with chromatin, affecting its form and compaction, are considered to be components of the nuclear matrix. In other words, the nuclear matrix is not simply a passive structure that objects can attach to. It consists of active agents — and, in our current context, that means agents of gene regulation.
The human genome contains an estimated 30,000-80,000 "matrix attachment regions" (MARs) — relatively short DNA sequences susceptible of being anchored to the nuclear matrix (Ottaviani et al. 2008). These anchoring points can contribute to the formation of the loops we've been talking about. Some MARs are more or less permanently attached to the matrix and may be associated with higher-order chromatin compaction and the repression of genes not required in a particular cell type. Others only transiently attach to the matrix and are thought to play a major role in the management of gene expression. The configuration of attachments at any given moment shapes the overall chromosome architecture, and the consequent looping patterns effectively insulate some regions from regulatory factors while exposing others. In sum:
Our understanding of how the genome functions in the context of the nucleus has been propelled by indisputable evidence that distinct genomic sites bind to regulatory proteins at the nuclear matrix. The emerging picture is that these genomic anchors regulate transcription and replication by dynamically organizing chromatin in three-dimensional space. (Ottaviani et al. 2008)
By this time I'm sure you recognize the need to ask (upon hearing that genomic anchors "regulate transcription"): What is it that regulates the anchors? How do they know when and where and for how long to participate in their anchoring task? The point, which is one of our enduring themes in these articles, is that there is no single, controlling level of gene regulation, subordinating the rest of the organism to itself. Noting that "the existence of regulatory cross-talk between spatially interacting loci opens up a new dimension in the study of gene regulation", Christian Lanctôt et al. (2007) go on to remark that "not only does [this cross-talk] constitute an additional level of complexity in the search for regulatory elements in the genome, it also implies that chromatin mobility itself, and therefore the ensuing long-range gene-gene interactions, might be a target of regulation".
How could it be otherwise within a harmoniously functioning organism? I
suspect it's quite safe to say that every aspect of the cell is in one way
or another a target of regulation, and at the same time takes on some of
the role of regulator. Or better (since that last statement reduces the
word "regulation" to something close to nonsense): every part participates
in the whole organism and is informed by the whole. Of course, in the
current state of biology this remark, too, will strike many as nonsense.
One could reply by asking whether it's any more nonsensical than
all the usual talk of "regulation", but a more positive approach would be
to take up the question of holism, as we will do later in this series.
Nuclear Compartments and Organelles. Loops are created when separate points on a chromosome are brought together and at least temporarily bound at the same location. Multiple-loop structures result when a number of different loci fraternize in this way. This raises the question: in what sense are the regions where these gatherings occur "real places"? That is, what structural identity do they possess beyond the fact that they happen to be sites where active chromosomal loci have gathered?
In one sense, the answer is easy. In order for genes to be active, there must be transcribing enzymes and many other factors related to transcription. So it stands to reason that these centers of activity are distinctively constituted. High-resolution surveys of the cell nucleus do in fact show many such places, which have come to be called transcription "factories" (a rather prejudicial term). Estimates for their number range from 500 to 10,000 (Trinkle-Mulcahy 2008), and it has been conjectured that, on average, some eight transcribing enzymes are present in each center of activity.
One group of researchers, describing how distantly separated genes in red blood cells "colocalize to the same transcription factory at high frequencies", go on to summarize the situation: "active genes are dynamically organized into shared nuclear subcompartments and movement into or out of these factories results in activation or abatement of transcription. Thus, rather than recruiting and assembling transcription complexes, active genes migrate to preassembled transcription sites" (Osborne et al. 2004). The implication, noted by the authors, is that "mechanisms regulating recruitment of genes into factories would be expected to have a fundamental role in gene expression".
The transcribing enzymes in (or, rather, at the outer surface of) the active transcription centers, according to the emerging view, do not themselves move along the genes; they "reel in" the genes they are transcribing. In this way they act as critical structural elements for maintaining the loops, which come and go as the various enzymes and regulatory factors bind and release them (Carter et al. 2008).
But, still, uncertainty remains about how much "there" is really there in the transcription centers. To what degree do enduring structures exist apart from the organized "structure" of the ongoing processes of transcription? There is presumably something there, but it's proven subtle and difficult to pin down. The matrix attachment regions of chromosomes, which presumably play a role in bringing genes to transcriptional centers, are being identified, but it remains to find anything in the way of a very fixed and definite structure for them to attach to. The "structure", such as it is, seems to be as much process as product.
There are yet other nuclear compartments relating to gene expression, but we will not pursue them here.
The intricately formed activity of the nucleus varies from one cell type to another and from one stage of an organism's development to another. It both shapes and mirrors the distinctive character of the individual cell. But this character is not some abstract essence detached from whatever else is going on in the organism at a particular moment. We can only assume that, whether the cat we are looking at is stalking or eating or sleeping or raising its fur in a confrontation with an enemy, the expressive differences we can recognize in these activities would be matched by expressive differences at every level of the cat's life, including the level of gene transcription and nuclear organization — if only we were capable of reading the cell with the same qualitative attention we devote to the outward behavior of the cat. If the cat is raising its fur, then the skin, muscle, and other cells must in some sense be "raising their fur" as well.
In other words, the chromosome movements we've looked at are always part of the larger activity of the organism. It's not just that a locus of the chromosome moves from point A to point B in order to connect with a group of other loci; this process in turn takes place in order to achieve equally significant performances at higher levels of observation, whether it's a matter of the cell's response to a nearby lesion or to starvation or to the organism's emotional state. The activities in the cell nucleus are part of an overall organic picture, and the scientist will do well to remember occasionally how remote is the detailed knowledge of the sort I've outlined above from any coherent and contextual understanding of what's going on.
This is presumably why biologists Amy Hark and Steven Triezenberg (2001), speaking of the variety of protein complexes affecting chromatin structure and gene expression, point to "a web of functional interactions that might be viewed as either elegantly integrated or hopelessly tangled". Hopelessly tangled, that is, if we do no more than lose ourselves in tracking isolated "causal factors" and "effects"; elegantly integrated if we can somehow rise to a more pictorial and qualitative grasp of what clearly is in fact a unified whole.
Perhaps we have the most incentive to seek such a wider understanding when we confront disease. There is no doubt, for example, that the phenomena investigated by the epigeneticist bear heavily on cancer, even if there is little effort as yet to read the cell as an expressive whole. Certainly research into particular "mechanisms" is proceeding at full tilt. Referring to how the microenvironments of the nucleus bring together the various gene-regulatory signals in all their necessary combinations, one team of researchers reviews the implications for cancer diagnosis and treatment:
Solid tumours, leukaemias, and lymphomas show striking alterations in nuclear morphology as well as in the architectural organization of genes, transcripts, and regulatory complexes within the nucleus. . . . These cancer-related changes disrupt several levels of nuclear organization that include linear gene sequences, chromatin organization and subnuclear [compartments]. . . . Modifications in chromatin remodelling complexes, the persistent association of regulatory proteins with gene loci, and DNA methylation epigenetically modulate genome accessibility to regulatory factors for the physiological control of cell fate.... (Zaidi et al. 2007).The researchers add that the effects of therapeutic treatment hang in the balance of these complex interactions, since even a patient's sensitivity to radiation and chemotherapy depends on the "composition, assembly and architectural organization of regulatory machinery within the cancer cell nucleus". The hope, finally, is that the "functional relationship between nuclear organization and gene expression" will bring advances in "tumor diagnosis" and "therapeutic responsiveness".
That hope may sooner or later be fulfilled. But the scale of the challenge looks hard to underestimate!
In any case, the fluid spatial organization of the nucleus and the movement of chromosomes within it clearly play a vital role in bringing about the right "marriages" between participants in the intricate playing out of genomic expression — and also in avoiding the wrong marriages. The evidence suggests, according to UK geneticists Peter Fraser and Wendy Bickmore, that "the dynamic spatial organization of the nucleus both reflects and shapes genome function. . . . We now have a picture of a genome that is 'structured', not in a rigid three-dimensional network, but in a dynamic organization [that] clearly changes during normal development and differentiation" (Fraser and Bickmore 2007).
We began by asking ourselves how the cell condenses two meters of DNA into a nucleus ten millionths of a meter in diameter. The question is justified, but we can see by now that it's hardly a mere matter of avoiding a snarled state so that an autonomous logic of transcription can proceed along its fated way. The adroit dynamics and deft sculpturing of chromosomes and an entire galaxy of proteins are as much the "whole point of the show" as any fixed code. The logic of transcription itself is, at least in part, a disciplined art of movement. The next time you find yourself picturing heredity as the transmission of fixed, determinative elements from parent to offspring, you might pause to ask yourself how such statically imagined elements could determine the art of movement that also comes to expression in successive generations.
There is, after all, as much cause and effect, as much determination of outcomes, as much logic and reason, in the compaction and twisting, the movement and re-shaping, as there is in any other aspect of the cell nucleus, even if the dynamism is fluid and irreducible to digital terms. The chromosome performs an unceasing dance and — crucially — the ever-shifting pattern of the dance lends its form and organization to the expression of genes. Perhaps that is why a pair of geneticists could write — very wisely, I think — that trying to define the chromatin complex "is like trying to define life itself" (Grewal and Elgin 2007).
If we ignore the artful movement, it's not because we find in it little meaningful expression of the cell's nature, but only because we have a difficult time translating it into the familiar and preferred terms of science. But that's a limitation of our science, not of the cell. We already have enough evidence to say that the movement, as movement, must be at least as deft and graceful, and at least as well-calculated, as any Olympic gymnast's.
Do the genes control the cell and hand down instructions? Whatever reason there may be to view the matter from that angle, there's at least as much reason to think of the dance of chromosomes as controlling the genes. Which individual genes can be expressed and how much; which "signaling" functions of the cell are brought to bear on any particular gene; which large stretches of the chromosome are prepared for longer-term expression and which are put into "cold storage" — all this is not so much digitally enunciated as gestured by the entire context. And the choreography continually varies, summoning genes to participate in the power of its higher-order artistry.
It is not too much to say that the cell presents us with forms constantly modulated by the cellular environment and beyond — living sculptures, shape-shifting in response to a music we have not yet inquired about, let alone learned to hear.
(You will find the latest versions of the currently available parts of this series at the website, "From Mechanism to a Science of Qualities".)
Please Note: With a view toward the needs of the readership, I have preferred to cite review articles, where they are available and, in general, have made little effort to reflect in my citations the priority claims of the various investigators of any particular phenomenon. Public (online) accessibility of papers and ease of access to the relevant information are primary criteria for my selection — qualified, of course, by the limits of my own familiarity with the literature.
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Steve Talbott :: NetFuture #176 :: May 28, 2009
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